FAQS

Probably less than you think.  3’ Transcriptomics can be generated from 10pg RNA or 10 cells.  16S variable region metagenome can be generated from less than a nanogram of DNA and as little as a picogram.  Amplicon sequencing requires 0.5 ng DNA and bacterial genome sequencing requires 20pg to 1ng DNA.  More sample is not necessarily better, and for DNA sequencing quality is more important than quantity of sample.

LGF offers bioinformatics, computational biology, computational modeling, and machine learning applications to analyze your sequence data.

No problem.  LGF can assist with the analysis of your genomic, transcriptomic, proteomic, and metabolomic data even if it was not generated at the LGF.

The LGF does not archive user biological samples.  If possible, you should retain a portion of your DNA or RNA samples prior to giving them to the LGF.  The LGF can also return to you any unused sample or sequencing libraries, but the best time to make arrangements for this is before you drop off samples with the LGF.

The LGF does not archive user data for longer than is required for users to download their sequencing data.  We strongly recommend that users back up a copy of their sequencing data to either a cloud-based service or on a physical, external storage device.

Of course.  In fact, the LGF recommends that, when feasible, users generate their own sequencing libraries.

The LGF will be happy to assist users with their grant applications.

The LGF will be happy to provide you with a letter of support for your project.

The LGF has Illumina MiSeqs, capable of generating 25 million reads up to 600 bp long.  LGF can generate deeper sequencing by running sequencing libraries on multiple flowcells.

Please ask!  The LGF can work with you to generate new sequencing workflows appropriate for your experimental design and data requirements.