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Services

  • Consultation
    • Consult with principal investigators and graduate students to design experiments
    • Share existing protocols
    • Develop new protocols
  • Training
    • Training on all equipment
      • Zeiss Elyra 7 dual iterative lattice structured illumination microscopy (SIM2) system
      •  Leica TCS SP5 multiphoton/confocal microscope
      • Philips CM 120 transmission electron microscope
      • Leica EM UC7 ultramicrotome
      • Training of users on the preparation of samples for imaging by dual iterative lattice structured illumination microscopy (SIM2).
      • Training of users on the preparation of samples for imaging by confocal microscopy.
      • Training of users on the preparation of samples for imaging by transmission electron microscopy.
  • Sample Preparation
    • Preparation of cell culture and tissue specimens for transmission electron microscopy. This includes fixation, osmification, dehydration, en bloc heavy metal staining, and embedding in epoxy resin.
    • Preparation of virus, pseudovirus, protein, phage, extracellular vesicles, and synthetic nanoparticles for transmission electron microscopy. This includes incubating coated electron microscopy grids with the sample in solution then contrasting for subsequent imaging.
    • Preparation of specimens, including tissue, protein, and extracellular vesicles, for immunogold electron microscopy.
    • Preparation of specimens for correlative light and electron microscopy (CLEM) experiments.
    • Preparation of specimens by photoconversion microscopy for imaging by transmission electron microscopy.
  • Sectioning and Staining
    • Sectioning of thin (~1 µm) and ultrathin (25-90 nm) sections via a Leica EM UC 7 ultramicrotome. This includes conventional ultramicrotomy and serial sectioning.
    • Collection of thin sections, staining with toluidine blue, and imaging by light microscopy.
    • Collection of ultrathin sections, including serial sections, onto electron microscopy grids.
    • Staining samples with contrasting agents including lead citrate, uranyl acetate, and phosphotungstic acid.
    • Performance of standard histological stains.
  • Imaging Acquisition
    • Multi-color imaging of live cells and fixed specimens.
    •  Imaging specimens by transmission electron microscopy.
    • Single molecule localization microscopy (SMLM).
  • Image Analysis
    • Analysis of fluorescence by Zeiss Zen software, Leica Application Suite software, Oxford Instruments Imaris software, and Image J.
    • Analysis of 3D volumes of electron micrographs via ESPINA software.
  • Consultation
    • Consult with principal investigators and graduate students to design experiments
    • Share existing protocols
    • Develop new protocols
  • Training
    • Training on all equipment
      • Zeiss Elyra 7 dual iterative lattice structured illumination microscopy (SIM2) system
      •  Leica TCS SP5 multiphoton/confocal microscope
      • Philips CM 120 transmission electron microscope
      • Leica EM UC7 ultramicrotome
      • Training of users on the preparation of samples for imaging by dual iterative lattice structured illumination microscopy (SIM2).
      • Training of users on the preparation of samples for imaging by confocal microscopy.
      • Training of users on the preparation of samples for imaging by transmission electron microscopy.
  • Sample Preparation
    • Preparation of cell culture and tissue specimens for transmission electron microscopy. This includes fixation, osmification, dehydration, en bloc heavy metal staining, and embedding in epoxy resin.
    • Preparation of virus, pseudovirus, protein, phage, extracellular vesicles, and synthetic nanoparticles for transmission electron microscopy. This includes incubating coated electron microscopy grids with the sample in solution then contrasting for subsequent imaging.
    • Preparation of specimens, including tissue, protein, and extracellular vesicles, for immunogold electron microscopy.
    • Preparation of specimens for correlative light and electron microscopy (CLEM) experiments.
    • Preparation of specimens by photoconversion microscopy for imaging by transmission electron microscopy.
  • Sectioning and Staining
    • Sectioning of thin (~1 µm) and ultrathin (25-90 nm) sections via a Leica EM UC 7 ultramicrotome. This includes conventional ultramicrotomy and serial sectioning.
    • Collection of thin sections, staining with toluidine blue, and imaging by light microscopy.
    • Collection of ultrathin sections, including serial sections, onto electron microscopy grids.
    • Staining samples with contrasting agents including lead citrate, uranyl acetate, and phosphotungstic acid.
    • Performance of standard histological stains.
  • Imaging Acquisition
    • Multi-color imaging of live cells and fixed specimens.
    •  Imaging specimens by transmission electron microscopy.
    • Single molecule localization microscopy (SMLM).
  • Image Analysis
    • Analysis of fluorescence by Zeiss Zen software, Leica Application Suite software, Oxford Instruments Imaris software, and Image J.
    • Analysis of 3D volumes of electron micrographs via ESPINA software.